DROSHA and colonic neoplasm: These include growing colorectal cancer cells to high confluence, depletion of miRNAs in the context of DROSHA knockout cells, rescue of miRNAs in the context of DROSHA knockout cells, depletion of TNRC6, expression of NLS-AGO2, expression of NES-AGO2, colorectal cancer cells grown in 3-D culture to form tumor spheroids, and human colon tumor tissue (Figure 12).