V-SVZ Tsc2wt/wt and Tsc2mut/mut cells were subsequently cultured from Tsc2wt/wt and Tsc2mut/mutx Nestin-CRE-ERT2 neonatal mice and RNA sequencing performed to identify mechanisms that may be responsible for hamartoma and heterotopic neuron generation. Here, MAPK3 is linked to hamartoma.