TSC2 and tuberous sclerosis: Electroporation of Cre recombinase (CRE) and green fluorescent protein (GFP) plasmids into neonatal mice having wild-type (wt) or conditional (f) Tsc2 alleles was performed to mimic somatic Tsc2 biallelic inactivation (mut) and to generate a model of TSC hamartomas and brains analyzed at P30 and P60 (Figures 1A–1C).