SHROOM3 and glomerulosclerosis: These mice exhibited mild albuminuria and increased protein-to-creatinine ratio that was not observed in controls indicating an age-dependent phenotype with reduced Shroom3 expression.35 Although these phenotypes could be explained by the fact that Shroom3 heterozygous null mice exhibit reduced nephron number, similar hallmarks of glomerulosclerosis (podocyte loss, increased glomerular area, and mesangial expansion) were observed in 1-year-old Shroom3 knockdown mice, that were generated using RNA interference (RNAi).