(13) Using both a Leiden community detection algorithm (39) (Fig. 2A) and a hierarchical clustering method (31) (Supplemental Fig. 1) we clustered cells from each individual patient sample and annotated cell clusters based on the original gene cell identity markers provided by Durante et al. (13) In five out of eight primary tumor samples, we identified one or more clusters of hybrid cells (Fig. 2Band Supplemental Files 5) based on co-expression of tumor genes MITF, MLANA, DCT, TYR, GP100, and HTR2B, and macrophage genes CD45, CD14, CD163 (Fig. 2C–2E for patient UMM059. This evidence concerns the gene TYR and neoplasm.