In comparison with mock-transfected controls, the result showed that the translational levels of HIPK3 were significantly increased in KKU-213B CCA cells transfected with specific miR inhibitor (p < 0.05; Fig. 5f and g). On the other hand, the levels of HIPK3 protein were significantly decreased in KKU-100 CCA cell line transfected with specific miR-205-5p mimic (p < 0.05; Fig. 5h and i). This evidence concerns the gene HIPK3 and cholangiocarcinoma.