We utilized RT-qPCR to examine the expression of HIPK3 in three CCA cell lines (KKU-213B, KKU-100, and KKU-055) and an immortalized cholangiocyte cell line (MMNK1) prior to transfection to select an appropriate CCA cell line for functional study of HIPK3. Here, HIPK3 is linked to cholangiocarcinoma.