HIPK3 and cholangiocarcinoma: Staining intensity was measured, calculated using the H-score formula, and assessed using Image J. Both normal adjacent and CCA tissue showed a positive response to HIPK3 in IHC; however, the degree of the staining was higher in normal adjacent tissue (Fig. 6b) than in low-expression (Fig. 6c) and high-expression (Fig. 6d) CCA tissue.