We identified thrombospondin-1 (THBS1), associated with the most aggressive and invasive GBM tumors, as one of the most highly downregulated G⍺12 dependent genes in the tumor samples analyzed by DESeq2, demonstrating that THBS1 expression was decreased by 90% in G⍺12 KD tumors. The gene discussed is THBS1; the disease is neoplasm.