Cell lysates prepared from human SH-SY5Y neuroblastoma cells stably overexpressing human WT APP695 (SH-SY5Y/APP695) or SH-SY5Y cells overexpressing both APP695 and S (SH-SY5Y/APP695/S) were immunoprecipitated using anti-S protein antibody, and then APP and S proteins were determined using western blot (WB) with 6E10 (anti-N-terminal APP; Fig. 1D, left panel) or S protein antibody (anti-S; Fig. 1D, right panel); Clear interactions between cellular APP and S protein can be observed. The gene discussed is PROS1; the disease is neuroblastoma.