PRF1 and infection: The CD8+Tcm subset slightly contracted in vaccinated/pregnant mice in response to Tc infection (Fig. 3s), yet functional activation of CD8+Tcm, evidenced by 67%, 113%, and 76% increase in IFNγ, PRF1, and GZB-producing subsets, respectively, was primarily noted in vaccinated/infected (vs. infected) pregnant mice (Fig. 3t–v, p < 0.05).