By analyzing the transcripts of 82 target genes using a qPCR array, we identified that Ru/5-FU can downregulate AKT1 (RQ = 0.392) in CRC HCT116 cells, suggesting that disruption of protein kinase B (Akt) signaling is a molecular target for Ru/5-FU (Fig. 2A and Table S3). The gene discussed is AKT1; the disease is colorectal carcinoma.