Although Ru/5-FU did not change NF-κB p65 (pS536) expression (Fig. 3A, B), a reduction in NF-κB p65 (pS529) (Fig. 3C, D) expression was observed in Ru/5-FU-treated HCT116 CRC cells. Here, NFKB1 is linked to colorectal carcinoma.