The results showed that, treatment of H9c2 cells with 60 μM ISO for 24 h induced a significant up-regulation of the expression of cardiac hypertrophy markers, atrial natriuretic peptide (ANP) and myosin heavy chain β (β-MHC), and had no significant effect on cell viability (Additional file 1: Fig. S2). The gene discussed is NPPA; the disease is cardiac hypertrophy.