EPHB1 and colorectal carcinoma: We next engineered DLD1 CRC cells to overexpress eGFP alone, wildtype EPHB1, or each of the selected mutants by lentiviral transduction with a polycistronic lentiviral expression vector in which expression of EPHB1 and eGFP were uncoupled due to the presence of IRES elements (Additional file 1: Fig. S1).