Indeed, vimentin was strongly expressed in meningioma spheroids and tissues (average immuno scores of 4, and 3.75 for tissues and spheroids respectively) while expression of the epithelial marker E-cadherin was weak in spheroids and tissues (average immuno scores of 1.75, and 0.25 for tissues and spheroids respectively) (Fig. 6a, b). Here, VIM is linked to meningioma.