We used either neutral (N, pHe = 7.5 (RPMI), 7.7 (DMEM)) or acidic (A, pHe = 6.8 (RPMI and DMEM) culture conditions in the presence or absence of IFN-γ and studied PD-L1 expression in cancer cells to investigate the role of acidosis. This evidence concerns the gene IFNG and cancer.