According to KEGG enrichment results, multiple hub targets are involved in the signaling process of numerous signaling pathways, such as RELA is involved in the IL-17 signaling pathway, PI3K-Akt signaling pathway, TNF signaling pathway and PD-L1 expression and PD-1 checkpoint pathway in cancer, while TNF is involved in IL-17 signaling pathway and TNF signaling pathway. The gene discussed is TNF; the disease is cancer.