CD4 and neoplasm: To test this, we purified Foxp3EGFP− CD4+ Tconv cells or Foxp3EGFP+ CD4+ Treg cells by FACS from B16-F10-tumor bearing Foxp3EGFP-DTR mice and incubated them with congenically distinct naïve CD4+ Tconv cells in vitro (Fig. 2A, Fig. S2).