To understand in greater detail how inactivation of SOCS1 affects the state of transferred CD8+ T cells within the tumor, single-cell RNA–Seq (scRNA-Seq) (10x Genomics) was performed on CD45+ bead-selected cells isolated from B16-OVA tumors harvested 7 days following the transfer of either sgSocs1, sgPD-1, or sgOlf OT1s. The gene discussed is CD8A; the disease is neoplasm.