We combined a MS paradigm with a subsequent mTBI or plantar incision surgery and measured behavioural nociception and comorbid anxiety, as well as systemic modulators of pain such as calcitonin gene-related peptide (CGRP) and Substance P. Additionally, we examined gene expression changes in interleukin 1 beta (IL1β) and glial fibrillary acidic protein (GFAP) within the cerebellum for associations between neuroinflammation and pain. Here, GFAP is linked to Anxiety.