A series of SB screens for HCC have been performed in a variety of other conditions including nonalcoholic fatty liver disease (PTEN conditional knock-out model), hepatic steatosis-inducing diet, and chronic liver injury (CCl4 model) that identified distinct environmental signal pathways involved in HCC tumorigenesis (Tschida et al., 2017; Kodama et al., 2018; Riordan et al., 2018). Here, PTEN is linked to hepatocellular carcinoma.