In agreement with this, flow-cytometry analysis revealed that the frequency of intratumoral CD8+ T cells and the CD8+ T cells expressing IFN-γ in C5aR−/− mice returned to the levels of WT tumor-bearing mice, along with increased PD-1 expression after macrophage depletion, leading to increased tumor weight and worsening survival in C5aR−/− mice (Figures 5I and S7I). Here, PDCD1 is linked to neoplasm.