HK2 and glioblastoma: Next, to determine whether HIF-1α could regulate S/G deprivation-induced genes involved in glucose transporters and glycolytic pathway, we treated with HIF-1α inhibitor PX-478, which successfully suppressed its protein expression (Fig. S4A of the SI) and transcriptional activity (Fig. S4B of the SI), and showed that HIF-1α inhibition resulted in the decreased mRNA expression levels of GLUT1, GLUT 3, HK2, and PFKFB2 induced by S/G deprivation in GBM cells (Fig. 4F).