ROS-AMPK-HIF-1α signaling, activated by serine and glycine deprivation, induces the expression of genes for glucose uptake, glycolysis, and serine synthesis pathway, leading to enforced glucose-derived de novo serine and glycine biosynthesis, which is required for the proliferation and survival of GBM cells and brain tumor growth. Here, HIF1A is linked to glioblastoma.