These results indicate that AMPK-HIF-1α signaling-mediated SSP activation plays an important role in the clinical behavior of human GBM and reveal a significant correlation between the levels of AMPK pT172 and HIF-1α expression, the expression levels of PHGDH, PSAT1, and PSPH and the clinical aggressiveness of GBM. Here, PHGDH is linked to glioblastoma.