Analyses of quantitative real-time PCR confirmed that S/G deprivation significantly induced the mRNA expression levels of GLUT1, GLUT 3, HK2, and PFKFB2 in glioma stem cells (GSCs) and GBM cells, including U87MG, LN18, and A172 (Fig. 2A). This evidence concerns the gene PFKFB2 and glioblastoma.