PIM1 and Familial prostate cancer: To test this, we cultured prostate cancer cells (PC3LN4 or DU145) expressing vector (pCIP) or PIM1 (hPIM1) in complete media (CM, RPMI1640, 10% dialyzed FBS, 25 mM Glucose) or introduced nutrient stress by restricting access to glucose (depleted media, DM, RPMI1640, 10% dialyzed FBS) for 24 h, and relative cell death was measured using SyTox, a cell-permeable dye that penetrates compromised plasma membranes characteristic of dead cells.