The most significantly cellular component enriched pathways associated with immobilization were “bladder cancer”, “cardiac muscle contraction “, “protein digestion and absorption “, “cytokine-cytokine receptor interaction “, “legionellosis, adrenergic signaling in cardiomyocytes “, “thyroid cancer “, “glutathione metabolism “, “IL-17 signaling pathway “, and “TNF signaling pathway “. The gene discussed is TNF; the disease is thyroid cancer.