As such, we examined the expression of Arrdc3, and as positive controls the well-known TRP53 targets Pmaip1 (encodes NOXA), Bbc3 (encodes PUMA), and Cdkn1a (encodes p21), in isogenic AF47A Eμ-Myc lymphoma cells with a non-targeting sgRNA (NTsgRNA) [32] or made Trp53KO by CRISPR/Cas9 (previously validated [31]) after 6 and 24 h of treatment with nutlin-3a or etoposide (Figs. 2A, S3). This evidence concerns the gene BBC3 and lymphoma.