In our study, we found that PDIA6 regulated TGFBR1 expression in endometrial cancer, which in turn lead to attenuated activation of the downstream cascade of the TGF-β signaling pathway, suggested that PDIA6 promoted cell proliferation, migration and invasion by regulating the TGF-β signaling pathway through a classical SMAD-dependent pathway. Here, TGFBR1 is linked to endometrial cancer.