In this study, we screened seven gene biomarkers related to the prognosis of HCC from the perspective of oxidative stress, measured five targets expression (ENO1, NDRG1, NPM1, TXNRD1 and IL-33) of the seven gene biomarkers to investigate the reliability of the multi-index prediction in clinic, increasing the sensitivity and specificity of the predictive model and resulting in a significant increase in overall confidence. This evidence concerns the gene ENO1 and hepatocellular carcinoma.