As reported in representative images and relative histograms, SOX2 knockdown significantly reduced the number of tumor spheroids from a mean of 164.21 to 139.81 in CAL27 and from 200.15 to 147.65 in SCC15 cells (*p-value < 0.05, CAL27) (**p-value < 0.01, SCC15). Here, SOX2 is linked to neoplasm.