As expected, multiple transcription factors, including vitamin D receptor (VDR), zic family member 3 (ZIC3), sterol regulatory element binding transcription factor 1 (SREBF1/SREBP1), general transcription factor IIi (GTF2I/TFIII), nuclear factor kappa B subunit 1 (NFKB1/NFKB), replication initiator 1 (REPIN1/AP4), sp1 transcription factor (SP1), POZ/BTB and AT hook containing zinc finger 1 (PATZ1/MAZR), visual system homeobox 2 (VSX2/CHX10), and LIM homeobox 3 (LHX3), may work as positive regulators in the trisomy 21 group. This evidence concerns the gene ZIC3 and trisomy 21.