Interestingly, we found that UBA52+CD8+T cells C2 showed higher T cytotoxic scores, TUBA4A+CD8+T cells C3 demonstrated higher T exhaustion scores but lower T cytotoxic scores, while VIM+CD8+T cells C1 and TUBA1A+CD8+T cells C4 presented lower both scores (Figure 4G), indicating TUBA4A likely to be involved in CD8+T cell exhaustion, result in tumor immune escape. The gene discussed is UBA52; the disease is neoplasm.