MAP1LC3A and infection: In order to identify the T. gondii effector(s) responsible for mediating strain-specific differences in host ATG response to infection in human cells, we quantified GFP-LC3 recruitment phenotypes of 34 unique F1 progeny isolated from the type I × type III genetic cross (45, 46) using the image-based recruitment assay described in Fig. 1 (data listed in Table S1).