In vivo experiments, 19 days after drug intervention, we can see that the final synthesized nanocomplex has basically the same effect as the complex without Bmi-1 gene knockdown.It may be due to the small volume of tumor formation, and the two groups of drugs can effectively inhibit the growth of tumor in nude mice, leading to the insufficient effectiveness gap in the course of short-term action (6 times). The gene discussed is BMI1; the disease is neoplasm.