We therefore used another classic animal model of depression, the chronic restraint stress model, for further verification, and to characterize the alleviating effects of Zuotai and β-HgS on the mood-like behaviors of chronic restraint-stressed mice, including interventions on stress hormone, inflammation, BDNF, monoamine neurotransmitters, and hippocampal histology. Here, BDNF is linked to major depressive disorder.