Compared with the CM group, the results showed that the VSMCs cultured in OM significantly induced the VSMCs from contractile phenotype to osteogenic phenotype, as indicated by the decreased mRNA levels of α-SMA, SM22-α, Cnn1 and Smoothelin and the increased mRNA levels of Runx2 and BMP2 (Figures 5E–J). The gene discussed is RUNX2; the disease is ocular melanoma.