To confirm whether the Warburg effect was responsible for the progression of HCC induced by RFX6, RFX6‐OE cells and relevant controls were treated with 2‐deoxyglucose (2‐DG), a glycolysis inhibitor, at different concentrations of 0, 4 and 8 mM for 24 h. The gene discussed is RFX6; the disease is hepatocellular carcinoma.