To verify the facilitating role of CHKB-AS1 in drug resistance inducement, plasmid vectors to overexpress CHKB-AS1 and shRNAs to knockdown CHKB-AS1 were transfected into ccRCC cells, and the proliferative abilities of NVP-BEZ235 treated cells were detected accordingly (Additional file 2: Fig. S1B–I). This evidence concerns the gene CHKB and nonpapillary renal cell carcinoma.