The examination of live cell morphology via light microscopy and 3D holotomography microscopy revealed formation of massive cytoplasmic vacuolization in KRAS MT/p53 WT NSCLC cells, A549, and H460, but not in KRAS WT cells, after treatment with nutlin-3a (Fig. 3A, B; Suppl. Here, TP53 is linked to non-small cell lung carcinoma.