Western blotting was also used to detect ALOX5 protein levels in the 786-O, ACHN, Caki-1, OS-RC-2, and A498 human ccRCC cell lines and the control HK-2 cell line, ultimately revealing that this protein was upregulated in ccRCC cells relative to HK-2 cells (Figure 10B), consistent with results observed at the mRNA level. Here, ALOX5 is linked to nonpapillary renal cell carcinoma.