Specially, a mixture of probiotic containing Bifidobacterium breve DM8310, Lactobacillus acidophilus DM8302 and Lactobacillus casei DM8121 at a ratio of 1:1:1 could increase the fecal water content and ameliorate loperamide-induced constipation by regulating AQP3 expression in rat colon (Deng et al., 2018). The gene discussed is AQP3; the disease is constipation disorder.