The antibiotic mixtures of A, B, and D could induce mice depressive behavior and depression-related neurobiological factors changes (including the decrease of NE, 5-HT, and BDNF contents in the hippocampus and prefrontal cortex), which is consistent with the changes in CUMS mice and depressed individuals in related studies. The gene discussed is BDNF; the disease is depressive symptom measurement.