IHC staining confirmed increased levels of hypoxic proteins (HIF1α and CA9) in pseudopalisades of GBM specimens, consistent with previous reports.[9, 18] TAGLN was lowly expressed in other regions of GBM, while highly expressed in the pseudopalisades of GBM (Figure 2B; Figure S2C, Supporting Information). This evidence concerns the gene CA9 and glioblastoma.