Secondly, we quantified release of cytokines (i.e., IFNγ, IL-10, IL-2, IL-6, TNFα, GM-CSF, IL-12p70, IL-1b and IL-8) and granzyme B by using the MSD platform in supernatants collected from the TDCC assay with the tumor cell lines SK-CO-1, MKN-45 and H2122 after 48 h. The gene discussed is CSF2; the disease is neoplasm.