As for the regulation of EMT, the expression of epithelial marker, E-cadherin, in A549 and H1975 was upregulated after the knockdown of SPOCK1, while the expression of the mesenchymal markers, that is, N-cadherin, vimentin was downregulated; furthermore, it is well known that matrix metalloproteinases (MMPs) have an important role in tumor progression and metastasis [27], our results also indicated that knockdown of SPOCK1 significantly decreased the expression of MMP9 (Fig. 7C, D). This evidence concerns the gene CDH2 and neoplasm.