Notably, endothelial cell proliferation assay of HUVEC, cocultured with IL30-overexpressing or IL30 gene knockout DU145 and PC3 cells, confirmed that tumor-derived IL30 stimulates EC proliferation, which was suppressed by coculture with IL30KO-PC cells, as shown by confocal microscopy and automated quantitative image analysis of CD31+ECs cocultured with EpCAM+PC cells (Fig. 1F, G, H). The gene discussed is PECAM1; the disease is pachyonychia congenita.