Results of the MSP RT-PCR study revealed that while ZNF516, PLCB3, MBP, and FGR were found to be hypermethylated in NPC (ΔCTZNF516 = 0.5, ΔCTPLCB3 = 0.639, ΔCTMBP = 1.138 and ΔCTFGR = 0.733), HLA-DRB5 was hypomethylated. This evidence concerns the gene PLCB3 and nasopharyngeal carcinoma.