WT1 and cancer: With this approach, WT1-specific CD8+ T cells were detected in both thymus and peripheral tissues of these mice.200 Based on the precise sequencing of CDX or PDX, rapid construction of humanized mice using matched-HLA human PBMCs or hematopoietic stem cells allows for a more comprehensive immune system through strategies, such as thymus transplantation, which is still important for assessing the immunogenicity and tumor-suppressing efficiency of future neoantigen cancer vaccines.