Altered DNA methylation in regions of T2D candidates bound by islet-specific TFs, as seen for LRFN2 in this study, with increased methylation in a region bound by all studied TFs (FOXA2, MAFB, NKX2.2, NKX6.1, and PDX1), can be a mechanism leading to reduced islet expression in T2D and, thus, impaired insulin secretion25. This evidence concerns the gene NKX6-1 and type 2 diabetes mellitus.