Therefore, we crossed Cst7-/- mice with the AppNL-G-F model of amyloid-driven AD (Saito et al., 2014) to generate background-matched non-disease (AppWt/WtCst7+/+), disease (AppNL-G-FCst7+/+) and disease knockout (AppNL-G-FCst7-/-) mice. Here, CST7 is linked to Alzheimer disease.