Supplementation of the increased dose of cholecalciferol + calcidiol had the most significant effect on the estrogen signalling pathway (p-value = 8.74E-09), TGF-β signalling pathway (p-value = 2.69E-08), signalling pathways regulating pluripotency of stem cells (p-value = 1.80E-05), phosphatidylinositol signalling system (p-value = 2.45E-05) and proteoglycans in cancer (p-value = 6.42E-05). The gene discussed is TGFB1; the disease is cancer.