We found that among the 32 cytokines measured, there were eight proinflammatory cytokines that were significantly reduced in CF mice exposed to HBEC EV compared with control—namely, Granulocyte-Macrophage Colony-Stimulating Factor: GM-CSF (P = 0.04), IFN-γ (P = 0.03), IL-3 (P = 0.01), IL-15 (P = 0.01), Macrophage Inflammatory Protein: MIP-1β (P = 0.03), MIP-2 (P = 0.01), Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted: RANTES (P = 0.03), and TNF-α (P = 0.04). Here, TNF is linked to cystic fibrosis.