The mRNA expression of these genes in TCGA-PRAD samples was determined, and in both non-paired (Additional file 1: Fig. S1b) and paired samples (Additional file 1: Fig. S1c), HK2, HK3 and ADPGK were overexpressed, while GCK and HK1 were downregulated in tumor samples compared with normal tissues. This evidence concerns the gene HK2 and prostate adenocarcinoma.