To determine whether SIRT6 affects Delta16HER2-dependent mammary carcinogenesis, we generated Delta16HER2/SIRT6-OE mice by breeding the Delta16HER2 breast cancer model with the functionally competent Sirt6BAC mice52 which have a two-fold SIRT6-OE (Fig. 1A). This evidence concerns the gene SIRT6 and breast cancer.